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桐柏野生大豆种子粗蛋白质含量的测定
引用本文:张乃群,丁妍妍,王璟,贾津亚,韩琼. 桐柏野生大豆种子粗蛋白质含量的测定[J]. 南阳师范学院学报, 2012, 11(12): 47-49
作者姓名:张乃群  丁妍妍  王璟  贾津亚  韩琼
作者单位:南阳师范学院生命科学与技术学院,河南南阳,473061
基金项目:河南省科技厅项目(102102110159);河南省教育厅项目(2010A180018)
摘    要:为了探讨野生大豆蛋白质含量,对产自桐柏的8个野生大豆样品采用半微量凯氏法和消化一分光光度法进行蛋白质含量测定.试验结果显示,两种方法测定的结果一致.半微量凯氏法是适用范围广测定结果精确的经典方法,消化一分光光度法相对于半微量凯氏法简化了试验操作,缩短了分析时间,更便于普及应用.

关 键 词:野生大豆  半微量凯氏法  消化-分光光度法  粗蛋白质

Determination of crude protein of Tongbai Glycine Soja seeds
ZHANG Nai-qun,DING Yan-yan,WANG Jing,JIA Jin-ya,HAN Qiong. Determination of crude protein of Tongbai Glycine Soja seeds[J]. Journal of Nanyang Teachers College, 2012, 11(12): 47-49
Authors:ZHANG Nai-qun  DING Yan-yan  WANG Jing  JIA Jin-ya  HAN Qiong
Affiliation:(School of Life Sciences and Technology,Nanyang Normal University,Nanyang 473061,China)
Abstract:In order to explore fast and precise determine crude protein content of wild soybean, using semi-micro Kjeldahl method and digestion-spectrophotometry determine the 8 wild soybean samples collected from Tongbai. Test results show that,the two methods have the same determination results. Semi-micro Kjeldahl method has a wide range of applications and accurate determination results, while digestion-spectrophotometry simplifies the test operation,shortens the analysis time,it' s more convenient for popularize application.
Keywords:Glycine soja  semi-micro Kjeldahl method  digestion-spectrophotometry  crude protein
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