| 针禾属植物羽毛针禾基因组DNA提取方法的研究 |
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| 引用本文： | 邓必建,张霞,王绍明. 针禾属植物羽毛针禾基因组DNA提取方法的研究[J]. 广东教育学院学报, 2007, 27(3): 64-68 |
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| 作者姓名： | 邓必建 张霞 王绍明 |
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| 作者单位： | 石河子大学生命科学学院，石河子大学生命科学学院，石河子大学生命科学学院 新疆石河子832003，新疆兵团绿洲生态农业重点实验室，新疆石河子832003，新疆石河子832003，新疆石河子832003，新疆兵团绿洲生态农业重点实验室，新疆石河子832003 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) , 教育部春晖计划项目 |
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| 摘 要： | 传统的CTAB DNA提取法步骤多.较烦琐,DNA产率低,而且由于酚、单宁、色素、多糖很难完全去除,容易影响随机扩增多态、微卫星等标记工作的效率.采用SDS裂解法提取了针禾属植物幼嫩叶片的基因组DNA,所获得的DNA可用于PCR反应.并且在针禾属植物的研究中得到了较好的结果.
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| 关 键 词： | 针禾属植物 羽毛针禾 SDS法DNA提取 CTAB法DNA提取 随机扩增多态DNA 微卫星 |
| 文章编号： | 1007-8754（2007）03-0064-05 |
| 修稿时间： | 2006-12-25 |
The Study on Extraction Method of Genome DNA in Stipagrosits pennata |
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| DENG Bi-jian,ZHANG Xia,WANG Shao-ming. The Study on Extraction Method of Genome DNA in Stipagrosits pennata[J]. Journal of Guangdong Education Institute, 2007, 27(3): 64-68 |
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| Authors: | DENG Bi-jian ZHANG Xia WANG Shao-ming |
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| Affiliation: | 1. College of Life Sciences, Shihezi University, Shihezi, Xinjiang, 832003, P. R. China; 2. Key Laboratory of Oasis Eco-Agriculture, Xinjiang Construction Corps, Shihezi, Xinjiang, 832003, P. R. China |
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| Abstract: | The traditional CTAB DNA extract method is troublesome with many steps and the gross Genomic DNA extracted by it is very low.High content of phenolic terpenoids,tannin,pigments,polysaccharide,etc.usually affect the activity of Taq and lead to the failure of PCR reaction.Genomic DNA of tender leaf is extracted in SDS method.The genomic DNA can be applied as template DNA in PCR reaction.The results suggested that SDS procedure was suitable for Stipagrosits genome DNA extraction. |
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| Keywords: | Stipagrosits De Winter Stipagrosits pennata SDS DNA extraction CTAB DNA extraction Random Amplified Polymorphic DNA SSR |
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