| 基因敲除技术及其应用 |
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| 引用本文： | 黄敏.基因敲除技术及其应用[J].广东轻工职业技术学院学报,2009,8(4):11-14. |
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| 作者姓名： | 黄敏 |
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| 作者单位： | 广东轻工职业技术学院,食品与生物工程系,广东,广州,510300 |
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| 摘 要： | 基因敲除技术是建立在同源重组技术之上,可对基因组进行定位修饰的实验方法。本文简述了基因敲除技术的基本原理、打靶策略、筛选机制,在动植物和微生物中常用的基因敲除方法以及基因敲除的应用。
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| 关 键 词： | 基因敲除 同源重组 打靶策略 筛选机制 |
Technology of Gene Knockout and Its Application |
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| Huang Min.Technology of Gene Knockout and Its Application[J].Journal of Guangdong Industry Technical College,2009,8(4):11-14. |
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| Authors: | Huang Min |
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| Institution: | Huang Min(Department of Food and Bioengineering,Guangdong Industry Technical College,Guangzhou 510300,China) |
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| Abstract: | Gene knockout was built on homologous recombination,and might be positional modified on the genome.This paper introduced the principle,the targeting strategies,the screening mechanism and the commonly method in animals,plants and micro-organisms of gene knockout.The application of gene knockout was also prospected in this paper. |
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| Keywords: | Gene knockout homologous recombination targeting strategies screening mechanism |
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