| 在土壤杆菌ZX09中透明颤菌血红蛋白的表达提高索拉胶的产量 |
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| 引用本文： | Yun-mei CHEN Hai-yang XU Yang WANG Jian-fa ZHANG Shi-ming WANG. 在土壤杆菌ZX09中透明颤菌血红蛋白的表达提高索拉胶的产量[J]. Journal of Zhejiang University. Science. B, 2014, 15(11): 979-985. DOI: 10.1631/jzus.B1400123 |
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| 作者姓名： | Yun-mei CHEN Hai-yang XU Yang WANG Jian-fa ZHANG Shi-ming WANG |
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| 作者单位： | Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China |
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| 基金项目： | Project supported by the Fundamental Research Funds for the Central Universities of China （No. 30920130121013） and the National Natural Science Foundation of China （No. 31300111） |
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| 摘 要： | 研究目的：提高胞外多糖索拉胶的产量。创新要点：透明颤菌血红蛋白基因在质粒上表达，不加抗生素发酵生产索拉胶。研究方法：将透明颤菌血红蛋白基因构建到广谱质粒pCM158上得到重组质粒pCM158-vgb，重组质粒转入土壤杆菌ZX09中，使透明颤菌血红蛋白在土壤杆菌ZX09中表达。重要结论：在土壤杆菌ZX09中颤菌血红蛋白的表达解决了发酵过程中氧受限的问题，提高了宿主菌的呼吸速率、蔗糖酶活性和黏度，将发酵产物索拉胶的产量提高了30％。
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| 关 键 词： | 索拉胶 透明颤菌血红蛋白 土壤杆菌ZX09 蔗糖酶 呼吸速率 |
| 收稿时间： | 2014-04-29 |
Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09 |
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| Yun-mei Chen,Hai-yang Xu,Yang Wang,Jian-fa Zhang,Shi-ming Wang. Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09[J]. Journal of Zhejiang University. Science. B, 2014, 15(11): 979-985. DOI: 10.1631/jzus.B1400123 |
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| Authors: | Yun-mei Chen Hai-yang Xu Yang Wang Jian-fa Zhang Shi-ming Wang |
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| Affiliation: | 1. Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, 210094, China
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| Abstract: | Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity. |
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| Keywords: | Salecan Vitreoscilla hemoglobin Agrobacterium sp. ZX09 Invertase Respiration rate |
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