神经精神科常用药物的快速定性定量及临床应用

目的：建立一种快速测定神经精神科常用药物的方法，并讨论其临床应用范围。方法：将前述两类药物按保留时间的长短分成两组，保留时间长的采用甲醇-水-1mol／L醋酸铵-5mol／L氨水（425／75／5／1V／V／V／V）的混合液做流动相，保留时间短的采用甲醇-水-1mol／L醋酸铵-5mol／L，氮啦（300／200／5／1V／V／V／V）的混合液做流动相。紫外检测，以外标法定量，实际应用时采用本院需检测病人的肘静脉血经处理后进样测定。结果：前递药物分离良好。线性相关系数大于0.996。回收率大于90％，日内、日间变异度均〈6.27％，实际应用效果良好。结论：此方法简便快速，适用于误服、中毒及自杀患者所用相关药物的定性与定量，鉴别副作用和药物过量、病情变化等。

Rapidly Determination of Usual Medicines Used in Neurology and Psychiatry & Clinical Application

OBJECTIVE:To establish a method for rapidly determination of Usual Medicines Used in Neurology and Psychiatry in serum with HPLC and discuss its clinical application patterns. METHODS:We divided these medicines to two group's according to their keeping time,the long keeping time group's mobile phase is consisted of Methanol-Water-1mol/Lammonium Acetate-5mol/ LAmmonia(425/75/5/1 V/V/V/V),the short keeping time group smobile phase is consisted of Methanol-Water-1mol/Lammonium Acetate-5mol/ LAmmonia(300/200/5/1 V/V/V/V),using UV- detector,medicine levels was determined by means of external standard method.Those needing checked patient s elbow vein blood was taken and the serum medicine levels were determined.RESULT:These medicines wereseparated well simultaneously.The coefficients of the linear equation were larger then 0.996. The rates of recovery were larger then90%. The variation coefficients of the between-day and with-day assay were less then6.27%. pratical using is good.CONCLUSION:The method is simple and rapid. It can be used to detect what medicines a commid suicide patient taked and how many he taked, whether a patient has mistaken some medicine,and a patient's symptoms is expressing of deleterious effect or intoxication or progress of the ill.