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COMPARISON OF DIFFERENT ENZYMES AND PROBES AND THEIR COMBINATIONS IN DNA FINGERPRINTING
引用本文:傅衍,牛冬,阮晖,陈海燕. COMPARISON OF DIFFERENT ENZYMES AND PROBES AND THEIR COMBINATIONS IN DNA FINGERPRINTING[J]. Journal of Zhejiang University. Science. B, 2001, 0(4)
作者姓名:傅衍  牛冬  阮晖  陈海燕
摘    要:INTRODUCTIONDNAfingerprintingisamethodforproducingtheDNAfragmentbandpattern ,specificforthegenomeofacertainindividual.Theproductionoftheindividualspecificbandpatternisbasedonthesimultaneousdetectionofseveralhighlypolymorphic,repetitivegenomicregions,wh…


COMPARISON OF DIFFERENT ENZYMES AND PROBES AND THEIR COMBINATIONS IN DNA FINGERPRINTING
FU Yan,NIU Dong,RUAN Hui CHEN Haiyan,Ponsuksili S. ,Horst P. .. COMPARISON OF DIFFERENT ENZYMES AND PROBES AND THEIR COMBINATIONS IN DNA FINGERPRINTING[J]. Journal of Zhejiang University. Science. B, 2001, 0(4)
Authors:FU Yan  NIU Dong  RUAN Hui CHEN Haiyan  Ponsuksili S.   Horst P. .
Affiliation:FU Yan,NIU Dong,RUAN Hui CHEN Haiyan,Ponsuksili S. 3,Horst P. 3.
Abstract:In the present study, eight combinations of restriction enzymes and oligonucleotide probes were tested for detecting VNTR polymorphism. More than a hundred loci were detected by all enzyme-probe combinations. The influences of breed, enzyme and probe as well as their interactions were analysed, and the mean value of DNA fingerprint data was calculated for the enzymes and probes. The results will provide some valuable information for studying the genetic relationship of individuals or populations using DNA fingerprinting.
Keywords:DNA fingerprinting   restriction enzyme   oligonucleotide probe   chicken
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