| IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS |
| |
| 作者姓名： | 陈智 刘勇 刘克洲 窦骏 |
| |
| 作者单位： | CHEN Zhi \ 1，LIU Yong \ 1，LIU Ke-zhou\ 1，Dennin R.H\ 2.，Dou Jun\ 1，Reinhard U\ 2(\{\}\ 1 Institute of Infectious Diseases，Medical College，Zhejiang University，Hangzhou 310003，China |
| |
| 摘 要： | ＩＮＴＲＯＤＵＣＴＩＯＮＲｉｂｏｚｙｍｅｗａｓｆｉｒｓｔｄｅｓｃｒｉｂｅｄｂｙＣｅｃｈａｎｄＡｌｔｍａｎｉｎ 1 981 .ＴｈｉｓｆｏｒｍｏｆＲＮＡｈａｄｈｉｇｈｌｙｓｐｅｃｉｆｉｃｅｎｄｏｎｕｃｌｅａｓｅａｃｔｉｖｉｔｉｅｓｔｏＲＮＡ (Ｋｒｕｇｅｒｅｔａｌ.,1 982 ) .Ｒｉｂｏｚｙｍｅｓｈａｖｅｔｈｒｅｅｓｔｒｕｃｔｕｒｅｓ:ｈａｍｍｅｒｈｅａｄ ,ｈａｉｒｐｉｎａｎｄａｘｅｒｉｂｏｚｙｍｅｓ.Ａｍｏｎｇｔｈｅｍ ,ｔｈｅｈａｍｍｅｒｈｅａｄｒｉｂｏｚｙｍｅｈａｓａｓｉｍｐｌｅｒｓｅｃｏｎｄａｒｙｓｔｒｕｃ…
|
IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS |
| |
| CHEN Zhi \ ,LIU Yong \ ,LIU Ke-zhou\ ,Dennin R.H\ .,Dou Jun\ ,Reinhard U\.IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS[J].Journal of Zhejiang University Science,2001(1). |
| |
| Authors: | CHEN Zhi \ LIU Yong \ LIU Ke-zhou\ Dennin RH\ Dou Jun\ Reinhard U\ |
| |
| Institution: | CHEN Zhi \ 1,LIU Yong \ 1,LIU Ke-zhou\ 1,Dennin R.H\ 2.,Dou Jun\ 1,Reinhard U\ 2 |
| |
| Abstract: | Objective: To determine the effective nucleotide sites of ribozymes against HCV , and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5'NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt.CD-SR (, containing either HCRZNC or HCRZC were constructed and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hybridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together. |
| |
| Keywords: | ribozyme inhibition hepatitis C virus MT-2 cells |
| 本文献已被 CNKI 等数据库收录！ |
|
