Optofluidic in situ maskless lithography of charge selective nanoporous hydrogel for DNA preconcentration |
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Authors: | Kim Hyoki Kim Junhoi Kim Eun-Geun Heinz Austen James Kwon Sunghoon Chun Honggu |
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Institution: | 1School of Electrical Engineering and Computer Science, Seoul National University, San 56-1, Shillim 9-dong, Gwanak-ku, Seoul 151-744, Republic of Korea;2Advanced Institutes of Convergence Technology, Seoul National University, 864-1, Iui-dong, Yeongtong-gu, Suwon, Gyeonggi-do 443-270, Republic of Korea |
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Abstract: | An optofluidic maskless photopolymerization process was developed for in situ negatively charged nanoporous hydrogel poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid)] fabrication. The optofluidic maskless lithography system, which combines a high power UV source and digital mirror device, enables fast polymerization of arbitrary shaped hydrogels in a microfluidic device. The poly-AMPS hydrogel structures were positioned near the intersections of two microchannels, and were used as a cation-selective filter for biological sample preconcentration. Preconcentration dynamics as well as the fabricated polymer shape were analyzed in three-dimensions using fluorescein sample and a confocal microscope. Finally, single-stranded DNA preconcentration was demonstrated for polymerase chain reaction-free signal enhancement. |
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