Cell rotation using optoelectronic tweezers |
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Authors: | Liang Yuan-Li Huang Yuan-Peng Lu Yen-Sheng Hou Max T Yeh J Andrew |
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Institution: | 1Institute of Electronics Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan;2Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan;3Department of Mechanical Engineering, National United University, Miaoli 36003, Taiwan |
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Abstract: | A cell rotation method by using optoelectronic tweezers (OET) is reported. The binary image of a typical OET device, whose light and dark sides act as two sets of parallel plates with different ac voltages, was used to create a rotating electric field. Its feasibility for application to electrorotation of cells was demonstrated by rotating Ramos and yeast cells in their pitch axes. The electrorotation by using OET devices is dependent on the medium and cells' electrical properties, the cells' positions, and the OET device's geometrical dimension, as well as the frequency of the electric field. |
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