金银花中挥发性成分的GC／MS分析及其指纹图谱的建立

研究了金银花挥发油的提取方法及其GC／MS的分析方法，通过气相色谱一质谱联用技术（GC—MS）分析了金银花挥发油的主要化学成分及其相对含量．建立了金银花的GC／MS指纹图谱，并利用该指纹图谱比较了两个不同产地金银花的质量情况．气相色谱／质谱法的实验条件是：毛细管柱为Rtx（30m×0．25mm×0．25μm）；进样口温度为250℃；程序升温为60℃至150℃（5℃／min），150℃至250℃（8℃／min）；60℃以后停3min，150℃以后停8min，250℃以后停5min,E1源温度200℃，离子源接口温度250℃．结果表明，金银花挥发油的提取率随地区的不同而不同，采用GC／MS法控制药材的指纹图谱，该方法重现性好、准确度高，可用于金银花的质量评价．

Fingerprint Analysis of the Volatile Chemical Constituents in Honeysuckle by Gas Chromatography/Mass Spectrum

The paper has studied the method of extracting essential oils in Honeysuckle and its main chemical constituents and relative contents by GC/MS. In order to compare the qualities of Honeysuckle in different yields, it is necessary to establish Gas Chromatography-Mass Spectrum fingerprints of its. The experimental condition of GC-MS, capillary mast： Rtx （30 m, 0.25 mm, 0.25μm ）; injection port temperature 250 ℃; process temperature： 60 ℃ to 150 ℃（ 5 ℃/min）, 150 ℃ to 250 ℃ （ 8 ℃/min ）; stop 3min since 60℃, stop 8 min since 150 ℃, stop 5 min since 250℃; EI resource： ion resource temperature 200 ℃, ion resource＇s injection port temperature 250 ℃. The result indicated that the volatile oil in Honeysuckle have different compositions following different fields. Consequently, this GC/MS fingerprints have a great repeatability and feasibility in analysis of Honeysuckle, and can be used in quality assessment of Honeysuckle.