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Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity
Authors:Wang Qing-bin  Xu Wen  Xue Qing-zhong  Su Wei-ai
Affiliation:(1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejon, 305-701, South Korea;(2) Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong 52, Yuseong, Daejon, 305-806, South Korea;(3) Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong 52, Yuseong, Daejon, 305-806, South Korea;(4) National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan;
Abstract:Transgenic Brassica compestris L. spp. chinensis plants expressing a choline oxidase (codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation. In the transgenic plants, codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (ELISA) examination, immunogold localization, and 1H-nuclear magnetic resonance (1H-NMR). Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions. The plants of transgenic line 1 (L1) showed significantly higher net photosynthetic rate (P n) and P n recovery rate under high (45 °C, 4 h) and low temperature (1 °C, 48 h) treatments, and higher photosynthetic rate under high salinity conditions (100, 200, and 300 mmol/L NaCl, respectively) than the wild-type plants. The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine, which is not found in the wild-type plants. Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L. spp. chinensis could be a potential strategy for improving the plant tolerance to multiple stresses.
Keywords:Brassica compestris L. spp. chinensis   codA   Stress   Glycine betaine   Net photosynthetic rate (Pn)
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