| 变色酸2B光度法测定血清蛋白质的研究 |
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| 引用本文： | 张永花,胡秋娈. 变色酸2B光度法测定血清蛋白质的研究[J]. 洛阳师范学院学报, 2005, 24(2): 55-58 |
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| 作者姓名： | 张永花 胡秋娈 |
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| 作者单位： | 洛阳师范学院化学系,河南,洛阳,471022 |
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| 摘 要： | 在pH1.62的Clark-Lubs(C-L)缓冲介质中,变色酸2B能与血清蛋白质形成有色复合物,其λmax为579nm,比试剂本身红移约65nm,表观摩尔吸光系数为2.09×105L·mol-1·cm-1(HSA),线性范围为0-100mg·L-1.研究了该反应体系的影响因素,确定了最佳反应条件,应用于人血清样品总蛋白的测定,结果与经典的考马斯亮蓝G-250法一致,而且线性范围宽,基本无干扰,操作与重现性都优于考马斯亮蓝法.
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| 关 键 词： | 光度法 蛋白质 变色酸2B |
| 文章编号： | 1009-4970(2005)02-0055-04 |
| 修稿时间： | 2005-01-05 |
Spectrophotometric Determination of Serum Proteins by Using Chromotrope 2B |
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| ZHANG Yong-hua,HU Qiu-luan. Spectrophotometric Determination of Serum Proteins by Using Chromotrope 2B[J]. Journal of Luoyang Teachers College, 2005, 24(2): 55-58 |
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| Authors: | ZHANG Yong-hua HU Qiu-luan |
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| Abstract: | Chromotrope 2B could bind with serum proteins to form a color complex in Clark-Lubs (C-L)buffer medium pH1. 62, which presents a maximum absorption at 579nm with 65nm of red shift compared to Chromotrope 2B itself. A molar absorptivity of 2.09 × 105 L·mol-1·cm-1(HSA) and linear range of 0 - 100mg/L for protein were determined. This method has been used for the determination of total protein in human serum samples. The results obtained by this method agreed well with those obtained by Coomassie brilliant blue G-250 method. Furthermore, the performance and reappearing are superior to Coomassie brilliant blue G-250 method. |
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| Keywords: | spetrophotometry protein chromotrope 2B |
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