Efficient dielectrophoretic cell enrichment using a dielectrophoresis-well based system |
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| Authors: | Mohd Azhar Abdul Razak Kai F Hoettges Henry O Fatoyinbo Fatima H Labeed Michael P Hughes |
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| Institution: | 1.Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom;2.Infocomm Research Alliance, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia;3.Deptech Ltd., 2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, East Sussex TN22 1QQ, United Kingdom |
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| Abstract: | Whilst laboratory-on-chip cell separation systems using dielectrophoresis are increasingly reported in the literature, many systems are afflicted by factors which impede “real world” performance, chief among these being cell loss (in dead spaces, attached to glass and tubing surfaces, or sedimentation from flow), and designs with large channel height-to-width ratios (large channel widths, small channel heights) that make the systems difficult to interface with other microfluidic systems. In this paper, we present a scalable structure based on 3D wells with approximately unity height-to-width ratios (based on tubes with electrodes on the sides), which is capable of enriching yeast cell populations whilst ensuring that up to 94.3% of cells processed through the device can be collected in tubes beyond the output. |
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