UV-B Induced stimulation of phosphatidylinositol 3-kinase in human fetal hepatocytes |
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| Authors: | V B Chari R S R Gaddameedi M Raghuveer Singh Sathish Kasina Khaja Khaleel Ur Rahman C M Habibullah Surya S Singh |
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| Institution: | (1) Department of Biochemistry, Osmania University, Hyderabad;(2) Center for Liver diseases, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad;(3) Department of Biochemistry, University College of Science Osmania University, 500 007 Hyderabad, India |
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| Abstract: | We investigated the effect of UV-B irradiation on PI 3-kinase activity in human fetal hepatocytes. When cells were exposed
to monochromatic (304nm) UV-B light, a significant increase in intracellular PI 3-kinase activity was observed in a dose dependent
manner with maximal activity upon 1500 Jm−2 irradiation. At 1500 Jm−2 dose PI 3-kinse activity increased by 80% in membrane fraction of fetal hepatocytes of 25 weeks gestation. PI 3-kinse inhibitors
wortmannin and LY294002 specifically inhibited the UV-B induced lipid kinase activity and blocked significantly the UV-B induced
cell viability. The data suggests a correlation between cell survival and elevated levels of PI 3-kinase and suggest that
UV-B irradiation at a dose of 1500 Jm−2 is ideal for fetal hepatocyte transplantation. Also, PI 3-kinase levels could be a representative marker for viable UV-B
irradiated fetal hepatocytes for transplantation. |
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| Keywords: | Signal transduction Lipid kinase Hepatocytes transplantation Xenotransplantation |
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