| 植物启动子克隆技术 |
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| 引用本文： | 尹振君,王秋平,吴延东,李合祥,田景汉.植物启动子克隆技术[J].实验技术与管理,2011(8). |
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| 作者姓名： | 尹振君 王秋平 吴延东 李合祥 田景汉 |
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| 作者单位： | 沧州职业技术学院组培中心;沧州师范学院后勤设备处;沧州师范学院生命科学系; |
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| 基金项目： | 国家自然科学基金项目(40971110) |
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| 摘 要： | 启动子作为基因表达调控的重要元件,成为分子生物学研究领域的热点。随着PCR技术的发展,从已知序列出发,克隆未知序列的方法日益成熟。综述了近年来植物启动子克隆技术的发展情况,介绍了基于PCR的染色体步行技术,包括:反向PCR(Inverse PCR)、随机引物PCR(randomly primed PCR)、连接介导PCR(ligation-mediated PCR)等方法,重点介绍了TAIL-PCR技术,并对该技术方法的优点及局限性进行了探讨。
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| 关 键 词： | 克隆技术 启动子 染色体步行 基因组 PCR |
Techniques of plant promoter cloning |
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| Yin Zhenjun,Wang Qiuping,Wu Y,ong,Li Hexiang,Tian Jinghan.Techniques of plant promoter cloning[J].Experimental Technology and Management,2011(8). |
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| Authors: | Yin Zhenjun Wang Qiuping Wu Y ong Li Hexiang Tian Jinghan |
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| Institution: | Yin Zhenjun1,Wang Qiuping2,Wu Yandong2,Li Hexiang2,Tian Jinghan3(1.Center of Tissue Culture,Cangzhou Vocational Technology College,Cangzhou 061001,China,2.Department of Logistics and Equipment,Cangzhou Teachers' College,3.Department of Life Science,China) |
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| Abstract: | As an important element for gene expression regulating,the promoter has become a hotspot in molecular biology researches.Along with the development of PCR techniques,the method of cloning unknown sequence from the known sequence is reaching maturity.This paper reviews the promoter cloning development in recent years,and introduces the techniques of chromosome walking based on the PCR amplification,including Inverse PCR,randomly primed PCR,and ligation-mediated PCR.This paper particularly emphasizes TAIL-PCR... |
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| Keywords: | promoter chromosome walking genomic PCR |
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