| 南阳牛血液基因组DNA提取与ANGPTL4基因的PCR扩增 |
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| 引用本文： | 马云,李芬,王启钊,裴灵芝,王伍.南阳牛血液基因组DNA提取与ANGPTL4基因的PCR扩增[J].平原大学学报,2009(2). |
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| 作者姓名： | 马云 李芬 王启钊 裴灵芝 王伍 |
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| 作者单位： | 信阳师范学院生命科学学院;苏州大学生命科学学院; |
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| 摘 要： | 采集20例南阳牛的血液,采用酚/氯仿法分离白细胞提取基因组DNA,并且对ANGPTL4基因部分序列(677～998 bp)PCR扩增条件进行了优化。结果表明,获得的牛基因组DNA经0.8%琼脂糖凝胶电泳检测,主条带清晰,表明获得的南阳牛基因组DNA可以用于后续实验;以所提取的基因组DNA为模板,优化ANGPTL4基因片段扩增的条件,结果表明,最理想的PCR扩增条件是模板量为2μL(50 mg/L),Taq聚合酶(5 u/μL)的量为0.8μL,退火温度为56℃,循环次数为35次。
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| 关 键 词： | DNA提取 ANGPTL4基因 条件优化 PCR |
Extraction of Genome DNA and PCR Amplification of ANGPTL4 Gene Segment for Nanyang Cattle |
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| MA Yun,LI Fen,WANG Qi-zhao,PEI Ling-zhi,WANG Wu.Extraction of Genome DNA and PCR Amplification of ANGPTL4 Gene Segment for Nanyang Cattle[J].Journal of Pingyuan University,2009(2). |
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| Authors: | MA Yun LI Fen WANG Qi-zhao PEI Ling-zhi WANG Wu |
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| Institution: | 1.College of life Science;Xinyang Normal University;Xinyang 464000;China;2.College of life Science;Soochow University;Suzhou 215006;China |
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| Abstract: | Blood samples were collected from 20 Nanyang catties and bovine genomic DNA were extracted by the way of phenol/chloroform extraction separation interleukin,and then the PCR expanding conditions of ANGTL4 gene's part sequence(from 677 to 998 bp) was optimized in this paper.The obtained bovine genomic DNA was detected by 0.8%agarose gel electrophresis and the clear bands was observed.The result showed that the genomic DNA we got can be used in the following experiment.The specific primer was designed and syn... |
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| Keywords: | DNA extraction ANGPTL4 gene condition optimize PCR |
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