| 酶-超声提取地黄多糖及其抗氧化性研究 |
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| 引用本文： | 李文波.酶-超声提取地黄多糖及其抗氧化性研究[J].德州学院学报,2009,25(4):46-50. |
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| 作者姓名： | 李文波 |
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| 作者单位： | 德州学院化学系,山东德州,253023 |
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| 摘 要： | 选用市售地黄为原料，利用纤维索酶解法结合热水浴提取法研究了提取温度、提取时间、提取液pH以及液料比对多糖提取量的影响．通过单因素试验得出地黄多糖提取工艺在液料比1：30的条件下的最佳参数为：提取温度40℃，提取时间25min，提取液pH为6．0，纤维素酶添加量为3．00％．在最佳参数组合下，多糖提取率为21．4％，地黄多糖对·OH的还原率为43．5％，此方法是简单、快捷和高效的提取方法．
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| 关 键 词： | 地黄 多糖 提取工艺 酶 |
Study on Polysaccharide Extracted from Radix Rehmanniae Using an Enzyme-ultrasonic Technique and Antioxidation |
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| LI Wen-bo.Study on Polysaccharide Extracted from Radix Rehmanniae Using an Enzyme-ultrasonic Technique and Antioxidation[J].Journal of Dezhou University,2009,25(4):46-50. |
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| Authors: | LI Wen-bo |
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| Institution: | LlWen--bo (Department of Chemistry, Dezhou University, Dezhou Shandong 253023, China) |
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| Abstract: | The polysaceharide was extracted from Radix Rehmanniae using the enzymatic hydrolysis for cel- lulose method and hot water bath method. The effects of four variables on the extraction quantity of polysaccharide were studied. Through a single-factor experiment, the optimum conditions of polysaccharide extraction under the ratio of sample to solvent 1：30 （m/V） were： extraction temperature 40℃, extraction time 25 rain. pH value of solvent 6.0, dosage of cellulase 3.0 %. Under these optimum conditions, the extraction rate of polysaccharide is 21.4 % and its degree of reduction to OH is 43.5%. It is a simple, convenient and efficient extraction method. |
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| Keywords: | Radix Rehmanniae polysaccharide extracts technique enzyme |
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