| 柑橘试管内微芽嫁接方法探讨 |
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| 引用本文： | 刘文龙,李进进,陈升云,翟华东,李志龙,李果安,黄维清,吴锦强,陈永炽,李海滔,卢欣. 柑橘试管内微芽嫁接方法探讨[J]. 广东轻工职业技术学院学报, 2009, 8(3): 14-16,20 |
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| 作者姓名： | 刘文龙 李进进 陈升云 翟华东 李志龙 李果安 黄维清 吴锦强 陈永炽 李海滔 卢欣 |
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| 作者单位： | 广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300;广东轻工职业技术学院食品与生物工程系,广东,广州,510300 |
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| 摘 要： | 目的：通过试管内微芽嫁接获得无病毒柑橘种苗。方法：用酸橙种子经消毒后在试管内播种作为砧木来源,采用还没有自剪的优良柑桔品种的嫩梢,取其一定大小的茎尖作为接穗进行试管内嫁接。结果：在种子消毒过程中,用75%酒精10s＋NaClO 8min＋HgCl210min的效果明显比其它三种消毒方法污染率低;在接种过程中,去除外壳的种子萌芽率最高;接穗的消毒用NaClO10min＋HgCl28min的消毒方法污染率较低。
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| 关 键 词： | 柑橘 接穗 微芽嫁接 |
Discussion on Orange Micro-shoots Grafting in Tube |
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| Liu Wenlong,Li Jinjin,Chen Shengyun,Zhai Huadong,Li Zhilong,Li Guoan,Huang Weiqing,Wu Jingqiang,Chen Yongchi,Li Haitao,Lu Xin. Discussion on Orange Micro-shoots Grafting in Tube[J]. Journal of Guangdong Industry Technical College, 2009, 8(3): 14-16,20 |
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| Authors: | Liu Wenlong Li Jinjin Chen Shengyun Zhai Huadong Li Zhilong Li Guoan Huang Weiqing Wu Jingqiang Chen Yongchi Li Haitao Lu Xin |
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| Affiliation: | Liu Wenlong,Li Jinjin,Chen Shengyun,Zhai Huadong,Li Zhilong,Li Guoan Huang Weiqing,Wu Jingqiang,Chen Yongchi,Li Haitao,Lu Xin(The Department of Food and Bio-technology Engineering,Guangdong Industry Technical College,Guangzhou 510300,China) |
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| Abstract: | Improved orange tree seeds without viruses were gained by micro-shoots grafting.The sour organge seeds which were sterilized were selected as stock scion source.Some fixed size of tender tops of good orange stems without self cuting were grafted in the tube.During the seeds sterilizing,the contaminate rate by using 75% alcohol 10s+NaClO 8 min+HgCl2 10 min was lower than other three sterilzing ways.While inoculations the contaminate rate by using NaClO 10 min+HgCl2 8 min was lower. |
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| Keywords: | orange scion micro-shoots grafting |
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