基因芯片技术及其数据处理方法

基因芯片又称DNA微阵列,该技术将大量的核苷酸探针以点阵列的方式排布于固相支持物上,与荧光标记的DNA样品杂交,通过激光共聚焦等方法对样品DNA进行定量检测。基因芯片技术具有自动化,高通量的特点,是基因表达谱研究的一种重要手段,在农业,医学,药物学等方面也有着广阔的应用前景。如何对基因芯片实验中所产生的大量数据进行有效的生物学分析,已成为芯片研究中的一个热点。基因芯片数据分析的流程通常包括了表达谱数据的获取与标准化,差异表达基因的筛选,基因的功能性分类及生物学意义的分析。

Genechip Techonology and Its Data Analysis

Genechip(DNA microarray) is such a techonology that all the nucleotide probes are arrayed on special supports and then hybridized with the target samples labeled with fluorescent dye,scanned with laser co-focus in order to measure the target DNA.The most striking features of the technology are automated and high-flux.It is widely used in the analysis of gene expression profile,and also has a brilliant prospect in the fields of agriculture,medicine and pharmaceutics.The methods how to efficiently analyse the biologic verification of the mass data generated by genechip experiment have become a hotspot in the chip research community.The data analysis of genechip includes the obtain and normalization of expression data,the selection of differential expression genes,the functional classification and biological interpretation of genes.