Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering |
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Authors: | Chun-mao Han Li-ping Zhang Jin-zhang Sun Hai-fei Shi Jie Zhou and Chang-you Gao |
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Institution: | 1.Department of Burn, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China;2.Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China |
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Abstract: | To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with
different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered
skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could
be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron
microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed
them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar
to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold
is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications. |
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Keywords: | Collagen Chitosan Fibrin glue Scaffold Tissue-engineered skin |
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