Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor |
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Authors: | Yang Yun-mei Cao Hong-cui Xu Zhe-rong Chen Xiao-ming |
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Institution: | (1) First Affiliated Hospital, College of Medical Science, Zhejiang University, 310003 Hangzhou, China |
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Abstract: | Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results: rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research. |
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Keywords: | Platelet activating factor Reversed phase high-performance liquid chromatographic technique Coronary heart disease Cerebral infarction |
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