| 澳洲袋鼠爪花种苗的组培快繁试验 |
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| 引用本文： | 周国权,陈樟烁,黄镇茂.澳洲袋鼠爪花种苗的组培快繁试验[J].嘉应学院学报,2012,30(11):56-59. |
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| 作者姓名： | 周国权 陈樟烁 黄镇茂 |
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| 作者单位： | 佛山市粤山生物科技有限公司,广东佛山,528000 |
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| 摘 要： | 以袋鼠爪花基部侧芽为外植体,进行了组织培养与快速繁殖技术的研究.结果表明:最佳诱导培养基为1/2 MS+BA 1.0 mg.L-1+NAA 0.05 mg.L-1+Ad 1‰+卡拉胶7 g.L-1+蔗糖20 g.L-1;最佳增殖培养基为1/2 MS+0.5 mg.L-1 BA+0.05 mg.L-1 NAA+Ad 1‰+卡拉胶7 g.L-1+蔗糖20 g.L-1;最佳生根培养基为1/2 MS+0.5 mg.L-1NAA+Ad 5‰+卡拉胶7 g.L-1+蔗糖20 g.L-1.炼苗后,移入泥炭和沙比例为2:1的基质中,移栽成活率高达90%.
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| 关 键 词： | 袋鼠爪花 组织培养 快速繁殖 |
Tissue Culture and Rapid Propagation of Anigozanthus Flavidus |
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| ZHOU Guo-quan,CHEN Zhang-shuo,HUANG Zhen-mao.Tissue Culture and Rapid Propagation of Anigozanthus Flavidus[J].Journal of Jiaying University,2012,30(11):56-59. |
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| Authors: | ZHOU Guo-quan CHEN Zhang-shuo HUANG Zhen-mao |
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| Institution: | (Foshan Yueshan Biotechnology Co.,Ltd.,Foshan 528000,China) |
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| Abstract: | The base bud of Anigozanthus flavidus was used as explants of tissue culture and rapid propagation in this study. Our results indicate that the optimal induced medium of callus induction was 1/2 MS medium with 1.0 mg·L^-1 BA, 0.05 mg·L^-1 NAA, 1‰ activated carbon, 7 g·L^-1 carrageenan and 20 g ·L^-1 sucrose. The op- timal medium for callus proliferation was 1/2 MS medium with 0.5 mg·L^-1 BA, 0.05 mg·L^-1 NAA, 1‰ acti- vated carbon, 7 g·L^-1 carrageenan and 20 g·L^-1 sucrose. The optimal medium for root differentiation was 1/2 MS medium with 0.5 mg·L^-1 NAA, 5 ‰ activated carbon, 7 g·L^-1 carrageenan and 20 g·L^-1 sucrose. After acclimatization, the seedlings were transplanted into base media which contained turf and sand （2 ： 1 ）, and the survival rate was beyond 90 %. |
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| Keywords: | Anigozanthus flavidus tissue culture rapid propagation |
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