The overshadowing of background stimuli by an informative CS in aversive Pavlovian conditioning with rats

Two experiments investigated the capacity of a nominal CS to overshadow background stimuli. Rats received CS-shock pairings in one compartment of a double-compartment apparatus. After training, the shock compartment was re-presented but in the absence of both the CS and shock, and overshadowing was assessed by measuring the rats’ latency to enter the compartment. In the first experiment, predictive values of temporal cues provided by CS onset and CS offset were manipulated. Rats readily entered the shock compartment, provided shock onset had previously been predicted by CS onset or CS offset. This indicates that background cues in the shock compartment had acquired little associative strength during training. In contrast, rats avoided the shock compartment under every condition in which shock onset had not previously been predicted by either CS onset or CS offset. In the second experiment, a precise temporal cue provided by CS onset was dissociated from other factors arising from CS-shock contiguity. It was found that rats’ reactions to the shock compartment after training was governed by both temporal and contiguity factors. The results are relevant to theories which state that conditioning is a function of a competitive relationship between stimuli present in an animal’s environment during training.