壳聚糖固定化血红蛋白氧载体的制备和性能研究

以固定化血红蛋白为核心的氧载体研究，有望为航空航天、水下作业以及好氧生物发酵工业等领域提供有效的供氧体系。以猪血红蛋白（pHb）和壳聚糖微粒为氧载体制备的基本原料，采用戊二醛活化将pHb共价固定在壳聚糖微粒上，构成pHb氧载体。研究了活化条件与载体表面醛基形成量及蛋白固定量之间的相互关系，对固定化反应进行了优化。在5％戊二醛浓度下活化反应8h，获得pHb最大固定量约800mg/g，即每克截体固定800mg pHb。从pHb氧载体的氧释放曲线可知，氧释放效率可达40％左右。该氧载体的结合稳定性较好。

Hemoglobin Immobilized on Chitosan as Oxygen Carriers

Oxygen carriers based on immobilized hemoglobin are promising in aviation, space flight, underwater work and aerobic fermentation. This paper studied the mechanism of porcine hemoglobin (pHb) immobilization on chitosan activated by glutaraldehyde. The influences of activation condition on aldehyde group content and on the amount of immobilized pHb were observed. The activation processes and conditions were optimized. By the activation procedure that the chitosan was treated with 5% glutaraldehyde for 8 hours, the carrier thus obtained was able to immobilize maximum amount of pHb, i.e. about 800mg /g chitosan. The oxygen carrier showed the highest oxygen release efficacy of 40% and good stability.