| 应用光热偏转光谱技术测量荧光素的绝对量子产率 |
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| 引用本文： | 吴惠桢,李正直.应用光热偏转光谱技术测量荧光素的绝对量子产率[J].科技通报,1991,7(4):185-187,206. |
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| 作者姓名： | 吴惠桢 李正直 |
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| 作者单位： | 杭州大学物理系 310028 |
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| 摘 要： | 应用光热偏转光谱技术(PDS)测量了荧光素Nile Blue和Naphthol Blue的绝对荧光量子产率;阐述了测量原理和实验方法。该技术与传统的光度测量方法相比,属交流信号测量,减少了干扰因素,具有调试、测量过程简便,系统误差小等特点,可用于新的发光标准的确定。
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| 关 键 词： | 光热偏转光谱 绝对量子产率 荧光素 |
Absolute Quantum Yield Determination by Photothermal Deflection Spectroscopy |
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| Wu Huizhen,Li Zhengzhi,Wang Zuo.Absolute Quantum Yield Determination by Photothermal Deflection Spectroscopy[J].Bulletin of Science and Technology,1991,7(4):185-187,206. |
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| Authors: | Wu Huizhen Li Zhengzhi Wang Zuo |
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| Institution: | Wu Huizhen,Li Zhengzhi,Wang Zuo,Department of Physics,Hangzhou University |
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| Abstract: | The photothermal deflection spectroscopy(PDS) is applied to absolute quantum yield determination for nile blue and naphthol blue. The principle and experimental results are described. The PDS is an alternating single testing technique. It has remarkable adrantages over conventional photometric method in that it can immu- nize from interference, and can simplify process of adjustment and measurement, and has less instrumental error. It is suitable to determine absolute quantum yield of new fluorescent standard. |
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| Keywords: | photothermal deflection spectroscopy absolute quantum yield nile blue naphthol blue |
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