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辣椒青枯病拮抗细菌的筛选及其鉴定
引用本文:葛红莲,赵红六,郭坚华.辣椒青枯病拮抗细菌的筛选及其鉴定[J].周口师范学院学报,2004,21(2):71-75.
作者姓名:葛红莲  赵红六  郭坚华
作者单位:1. 周口师范学院,生物系,河南,周口,466000
2. 周口师范学院,化学系,河南,周口,466000
3. 南京农业大学,植物病理系,南京,210095
摘    要:从河南扶沟、江苏南京和淮阴等地的辣椒、番茄和黄瓜植物根围土壤中分离到100个细菌菌株.采用抑菌圈-定殖力双重测定法,筛选出5株终定植密度大于104cfu/g根、抑菌能力较强的细菌菌株;并对5个生物防治菌进行了生理生化测定.结果表明,5个生物防治菌分别属芽孢杆菌属、沙雷氏菌属、假单孢菌属和肠杆菌属.

关 键 词:辣椒  青枯病  生物防治细菌  筛选  鉴定
文章编号:1671-9476(2004)02-0071-05
修稿时间:2003年11月12

Screening and identification for biocontrol agent against Ralstonia solanacearum
GE Hong-lian.Screening and identification for biocontrol agent against Ralstonia solanacearum[J].Journal of Zhoukou Normal University,2004,21(2):71-75.
Authors:GE Hong-lian
Institution:GE Hong-lian~
Abstract:One hundred bacterial isolates were obstained from the rhiszosphere of pepper,tomato and cucumber in vegtable-field soil of Nanjing and Huaiyin of Jiangsu Province and Fugou County of Henan Province from 2000 to 2001.From the isolates 5 strains with obvious inhibiting activity to pathogens and the root-colonizing density are higher than 10~(4)cfu/g root were screened as the biocontrol microbes by using the method of the inhibiting zones combined with root-colonizing capacity of the five isolates. The five biocontrol bacterial strains were identified according to the morphological ,physiological and biochemical characterestics. The identification result showed that R_(1), F_(3,)J_(1,)G_(6)and R_(6)were Baccilus sp.,Serratia sp., Pseudomonas sp.,_( ) Enterobacter sp., Erwinia sp.respectively.
Keywords:pepper bacteria wilt  biological bacteria  screening  indentification
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