Whole genome amplification in preimplantation genetic diagnosis |
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Authors: | Zheng Ying-ming Wang Ning Li Lei Jin Fan |
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Institution: | Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China. |
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Abstract: | Preimplantation genetic diagnosis (PGD) refers to a procedure for genetically analyzing embryos prior to implantation, improving
the chance of conception for patients at high risk of transmitting specific inherited disorders. This method has been widely
used for a large number of genetic disorders since the first successful application in the early 1990s. Polymerase chain reaction
(PCR) and fluorescent in situ hybridization (FISH) are the two main methods in PGD, but there are some inevitable shortcomings
limiting the scope of genetic diagnosis. Fortunately, different whole genome amplification (WGA) techniques have been developed
to overcome these problems. Sufficient DNA can be amplified and multiple tasks which need abundant DNA can be performed. Moreover,
WGA products can be analyzed as a template for multi-loci and multi-gene during the subsequent DNA analysis. In this review,
we will focus on the currently available WGA techniques and their applications, as well as the new technical trends from WGA
products. |
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Keywords: | Whole genome amplification Multiple displacement amplification Primer extension preamplification Degenerate oligonucleotide primed-polymerase chain reaction Preimplantation genetic diagnosis |
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