| 农杆菌介导的白菜遗传转化影响因子研究进展 |
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| 引用本文： | 范正琪,崔海瑞,王忠华,夏英武. 农杆菌介导的白菜遗传转化影响因子研究进展[J]. 金华职业技术学院学报, 2002, 2(1): 10-13 |
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| 作者姓名： | 范正琪 崔海瑞 王忠华 夏英武 |
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| 作者单位： | 1. 浙江大学原子核农业科学研究所,310029,杭州市
2. 金华职业技术学院生物工程学院 |
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| 摘 要： | 白菜遗传转化技术中最常用的方法是农杆菌介导法。由于白菜的再生能力较低，因此农杆菌介导白菜遗传转化的研究报道较少。本文就近十年来的研究进展作一简要回顾，并对影响其转化及再生频率的关键性因子，如植物基因型、外植体及生理状态、农杆菌菌株、感染方法和培养基附加成分等方面进行了较详细的讨论。
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| 关 键 词： | 农杆菌介导 白菜 遗传转化 影响因子 再生能力 外植体 培养基 |
| 修稿时间： | 2001-12-27 |
Advances in Genetic Transformation of Brassica campestris L. Aia by Agrobacterium-mediated Methods |
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| Fan Zhengqi Cui Hairui Wang Zhonghua Xia Yingwu. Advances in Genetic Transformation of Brassica campestris L. Aia by Agrobacterium-mediated Methods[J]. Journal of Jinhua College of Profession and Technology, 2002, 2(1): 10-13 |
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| Authors: | Fan Zhengqi Cui Hairui Wang Zhonghua Xia Yingwu |
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| Affiliation: | Jinhua College of profession and Technology |
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| Abstract: | The common method of Genetic Transformation of Brassica campestris L.is Agrobacterium-mediated method.The reports of this method in Brassica campestris L.can't often be seen because of its low shoot regeneration ability.We summarized the advances in Genetic Transformation of Brassica campestris L.by Agrobacterium-mediated Method since1995,and the main factors which affect this technique,such as genotypes of varieties,explants and its physiological state,Agrobacterium strains,method of infection,addition of medium,etc. |
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| Keywords: | Brassica campestris L.Agrobacterium-mediated method Genetic Transformation |
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