| 斑马鱼视网膜电图测量系统构建 |
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| 引用本文： | 刘亚丰,吴颖,卢群伟.斑马鱼视网膜电图测量系统构建[J].中国现代教育装备,2021(5):57-59,66. |
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| 作者姓名： | 刘亚丰 吴颖 卢群伟 |
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| 作者单位： | 华中科技大学生命科学与技术学院 |
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| 基金项目： | 湖北省2018年教学研究项目“虚实融合、自主设计、研究型实验教学模式探讨”(编号:201858);华中科技大学2019年教学研究项目“新时代生命科学拔尖人才培养模式的探索”(编号:2019029)。 |
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| 摘 要： | 视网膜电图可用于评判视觉系统的发育、功能及病变,已成为眼科必备检查项目之一。目前已开发商用系统仅用于人类眼科检查和大的动物研究,无法用于斑马鱼视网膜功能测量。利用玻璃电极、四维度微操、LED光刺激模块及电生理放大器,搭建了适合于幼小斑马鱼视网膜电图测量的系统,系统具有高信噪比、适应性强、方便操作、实验成功率高等优点,将在斑马鱼视觉系统发育、遗传学研究等方面发挥积极的作用。
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| 关 键 词： | 视网膜电图 电生理 斑马鱼 |
System for Measuring the Electroretinogram of Larval Zebrafish |
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| Liu Yafeng,Wu Ying,Lu Qunwei.System for Measuring the Electroretinogram of Larval Zebrafish[J].China Modern Education Equipment,2021(5):57-59,66. |
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| Authors: | Liu Yafeng Wu Ying Lu Qunwei |
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| Institution: | (College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan,430074,China) |
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| Abstract: | Electroretinogram is used to evaluate the development,function and pathology of visual system,which has become one of the necessary items in ophthalmology.At present,the commercial system is only used for human ophthalmic examination and bigger animal research,and cannot be used for retina function measurement on zebrafish.Here a system suitable for measuring electroretinogram of larval zebrafish was established,based on glass electrode,four-dimensional motorized manipulator,light stimulation module and electrophysiological amplifier.The system has the advantages of high signal-to-noise ratio,high adaptability,convenient operation,high success rate on experiment,and will play an important role in the research of the visual development and genetics of zebrafish. |
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| Keywords: | electroretinogram electrophysiology zebraifish |
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