| 对小球藻蛋白质的提取及分离的研究 |
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| 引用本文： | 林芃,刘艳,杨海波. 对小球藻蛋白质的提取及分离的研究[J]. 大连大学学报, 2002, 23(4): 70-73 |
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| 作者姓名： | 林芃 刘艳 杨海波 |
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| 作者单位： | 1. 大连大学,生物工程学院,辽宁,大连,116622
2. 大连大学,化学与化学工程系,辽宁,大连,116622 |
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| 基金项目： | 辽宁省科学技术基金资助项目（001059） |
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| 摘 要： | 为了研究小球藻中增强免疫力及抗肿瘤等方面具有显著效果的活性成分,本文对小球藻蛋白质的提取及分离进行了初步的研究.小球藻（Chiorella ssp）光照培养,离心收集鲜藻,超声波破碎细胞,离心除细胞碎片.取上清液加入30％饱和度硫酸铵,4℃静置盐析24h,离心后分别将沉淀和上清液透析,经Sephadex G-75柱层析,可以得到分子量不同的四种蛋白质,以PAGE分析证明了本文所采用的方法.
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| 关 键 词： | 小球藻 蛋白质 盐析 凝胶过滤层析 聚丙烯酰胺凝胶电泳 萃取 |
| 文章编号： | 1008-2395(2002)04-0070-04 |
| 修稿时间： | 2001-09-24 |
Study on extracting and fractionating of protein from chiorella spp. |
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| LIN Peng,LIU Van,YANG Hai-bo. Study on extracting and fractionating of protein from chiorella spp.[J]. Journal of Dalian University, 2002, 23(4): 70-73 |
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| Authors: | LIN Peng LIU Van YANG Hai-bo |
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| Abstract: | Chiorella spp. was cultivated for 8 days in at room temperature under fluorescent light. After 6 days, cells were collected by centrifugation, then disrupted by ultrasonic wave. After centrifugation, the supernatant was treated with 30% (NH4)2SO4 for 24 hour at 4℃. Again after centrifugation, the sediment and supernatant were dialyzed at 4℃ in distilled water for 24 hour. After desalination, each fraction was applied to Sephdex G-75 molecular sieve column, finally four proteins were obtained. The result was shown by PAGE. |
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| Keywords: | chiorella spp. protein salting out gel chromatography PAGE extract |
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