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Efficient gene editing in a medaka (Oryzias latipes) cell line and embryos by SpCas9/tRNA-gRNA
Authors:Pan  Qihua  Luo  Junzhi  Jiang  Yuewen  Wang  Zhi  Lu  Ke  Chen  Tiansheng
Institution:1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Fisheries College, Jimei University, Xiamen, 361021, China
;2.College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China
;
Abstract:Journal of Zhejiang University-SCIENCE B - Generation of mutants with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is commonly carried out...
Keywords:
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