力竭游泳对大鼠肠组织MDA、Free-SH含量和Na+-K+-ATPase活性的影响

为了探讨运动性肠功能紊乱的原因 ,将 2 4只 SD雄性大鼠随机分为 3组 ,即对照组 (C)、运动后 30 m in组 (EX1 )、运动后 6 0 m in组 (EX2 ) ,采用化学比色法测定力竭性游泳后 30 m in和6 0 min结肠组织 MDA、Free- SH含量和 Na - K - ATPase活性。结果显示 ,运动后 30 min和 6 0m in组 MDA含量显著增加 ,Free- SH含量显著下降 ,而运动后 6 0 m in组 Na - K - ATPase活性显著下降。结果提示 ,运动源性自由基产生增加使肠组织中游离巯基被氧化 ,导致 Na - K - AT-Pase活性下降 ,可能是造成运动性肠功能紊乱的重要因素。

A Study of Relation Between Exhaustive Swimming and MDA Free-SH Content,Na+-K+- ATPase Activity in Rat's Intestine Tissues

To investigate the cause of exercise induced intestine function disorder, 24 male SD rats were divided into three groups (control, 30min. post exercise and 60min. post exercise). We measure the contents of MDA, Free SH and Na K ATPase activity in rat's intestine tissues with chemistry colorimetry. The result showed that MDA content had significant increase at 30 min. and 60 min. after exhaustive swimming while Free SH content had a remarkable decrease. At the same time, Na K ATPase activity declined significantly 60 min. after exercise. These suggested that increase of exercise induced free radicals led to Free SH of intestine cross link, which resulted in the decline of Na K ATPase activity. These might be important causes of exercise-induced intestine disorder.