| 粉防己多糖的组成及其清除活性氧自由基的作用 |
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| 引用本文： | 甄攀,梁惠花,张万明,王治宝.粉防己多糖的组成及其清除活性氧自由基的作用[J].河北北方学院学报(医学版),2005,22(5):6-9. |
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| 作者姓名： | 甄攀 梁惠花 张万明 王治宝 |
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| 作者单位： | 河北北方学院化学教研室,河北,张家口,075000 |
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| 摘 要： | 目的:提取并纯化粉防己多糖(SMP),确定其组成,研究其清除活性氧自由基的作用.方法:利用水提醇沉得到粉防己粗多糖,再经DEAE纤维素和聚酰胺纯制.利用Fenton反应研究SMP清除·OH的作用;利用邻苯三酚自氧化法研究SMP清除·O2-的作用.结果:纸色谱显示SMP是由葡萄糖组成的匀多糖;SMP清除·OH的能力较弱;清除·O2-时的效果稍强,IC50为1956.1μg/ml.结论:粉防己多糖为首次提取制得,有一定的清除羟自由基和超氧阴离子自由基的作用.
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| 关 键 词： | 粉防己 多糖 抗氧化 羟自由基 超氧阴离子自由基 |
| 文章编号： | 1673-1484（2005）05-0006-04 |
Study on Chemical Composition and Antioxygenation of Polysaccharide from Stephania Tetrandra S. Moore |
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| ZHEN Pan,LIANG Hui-hua,ZHANG Wan-ming,et alHebei North University,Zhangjiakou,Hebei China.Study on Chemical Composition and Antioxygenation of Polysaccharide from Stephania Tetrandra S. Moore[J].Journal of Hebei North University:Medical Edition,2005,22(5):6-9. |
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| Authors: | ZHEN Pan LIANG Hui-hua ZHANG Wan-ming Hebei North University Zhangjiakou Hebei China |
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| Institution: | ZHEN Pan,LIANG Hui-hua,ZHANG Wan-ming,et alHebei North University,Zhangjiakou,075000,Hebei China |
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| Abstract: | Objective: To extract and purify Polysaccharide from Stephania tetrandra S. Moore(SMP),study on its chemical composition and the effect on scavenging active oxygen. Methods: The Polysaccharide was extracted by water and precipitated by ethanol.It was further purified by DEAE-cellulose and polyamide. Study Polysaccharide scavenging effect on ·OH with Fenton reaction. Study on Polysaccharide scavenging effect on ·O2- with Pyrogallol Autoxidation Method. Results: The paper chromatography showed that SMP was composed of glucose. The scavenging effect of SMP on ·OH was displeased. The IC50 of SMP scavenging ·O2- was 1956.1μg/ml. Conclusion: This Polysaccharide was isolated from Stephania tetrandra S. Moore for the first time, which had certain effect on scavenging ·OH and ·O2-. |
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| Keywords: | Stephania tetrandra S Moore Polysaccharide Antioxidation Hydroxyl radical Superoxide anion radical |
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