Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L. |
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| Authors: | Cheng-qi Yan Kai-xian Qian Gang-ping Xue Zhong-chang Wu Yue-lei Chen Qiu-sheng Yan Xue-qing Zhang Ping Wu |
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| Institution: | (1) College of Life Sciences, Zhejiang University, 310029 Hangzhou, China;(2) Ningbo Academy of Agriculture Sciences, 315040 Ningbo, China;(3) China National Rice Research Institute, 310006 Hangzhou, China;(4) CSIRO Plant Industry 306 Carmody Rd., St Lucia, 4067 Qld, Australia |
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| Abstract: | Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically
hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs.
Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology
of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with
highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.
Project (No. 98001) supported by Ningbo Agriculture Key Scientific Research Foundation, China |
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| Keywords: | Oryza sativa L Oryza meyeriana L Somatic hybridization Rice bacterial blight resistance |
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