| 响应面法优化苦参总黄酮的提取工艺 |
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| 引用本文： | 兰艳素,赵严,张婷婷,桂生,任文强.响应面法优化苦参总黄酮的提取工艺[J].黄山高等专科学校学报,2014(3):46-49. |
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| 作者姓名： | 兰艳素 赵严 张婷婷 桂生 任文强 |
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| 作者单位： | 黄山学院化学化工学院,安徽黄山245041 |
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| 基金项目： | 安徽省优秀青年人才重点项目（2013SQRL088ZD）;黄山学院自然科学研究项目（2011xkj017）;黄山学院大学生科研项目（2012xdkj073） |
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| 摘 要： | 采用微波法辅助提取苦参总黄酮,以乙醇体积分数、提取时间、液料比及微波功率为自变量,苦参总黄酮提取率为评价指标,采用Box-Behnken试验设计及响应面法分析优化提取工艺条件.结果表明,最优提取工艺为乙醇体积分数80％,提取时间6min,液料比25：1（mL/g）,微波功率400W.在此条件下,黄酮提取量达到12.67mg/g.
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| 关 键 词： | 响应面法 苦参 总黄酮 |
The Optimization of the Extraction of Total Flavonoids from Sophora Flavescenes by Response Surface Methodology |
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| Authors: | Lan Yansu Zhao Yan Zhang Tingting Gui Sheng Ren Wenqiang |
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| Institution: | (School of Chemistry and Chemical Engineering, Huangshan University, Huangshan245041, China) |
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| Abstract: | Microwave technology is applied to extract total flavonoids from sophora flavescenes. The process parameters are optimized by a Box-Behnken experimental design and response surface methodology with the concentration of ethanol, the extraction time, the ratio of liquid to material and the microwave power as four independent variables and the extraction efficiency of total flavonoids as evaluation index. The results indicate the optimum conditions of extraction process are as follows： the ethanol concentration is 80%, the extraction time 6 min, the ratio of liquid-to-solid 25：1 （mL/g） and the microwave power 400 W. Under these conditions, the yield of total flavonoids is up to 12.67mg/g. |
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| Keywords: | response surface methodology sophora flavescenes total flavonoids |
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