| 分离技术在蛋白质复性中的集成化应用及分析 |
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| 引用本文： | 崔志芳,关怡新,姚善泾.分离技术在蛋白质复性中的集成化应用及分析[J].科技通报,2004,20(2):99-104. |
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| 作者姓名： | 崔志芳 关怡新 姚善泾 |
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| 作者单位： | 浙江大学化学工程与生物工程学系,杭州,310027 |
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| 基金项目： | 国家自然科学基金资助项目(20276065) |
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| 摘 要： | 针对蛋白质复性这一基因工程蛋白质生产过程中的难点问题，着重介绍了一些蛋白质分离技术如超滤、反胶团萃取和凝胶萃取等在蛋白质复性及分离方面的集成化应用，并逐一分析了各自的作用机理及应用过程中所涉及到的一些共性及不同点，希望能够借鉴于相对成熟的蛋白质分离技术，促进蛋白质复性技术的进一步发展。
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| 关 键 词： | 生物化学工程 蛋白质复性 超滤 反胶团 凝胶萃取 |
| 文章编号： | 1001-7119(2004)02-0099-06 |
Integrated Applications of Several Separation Methods in the Protein Renaturation |
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| CUI Zhi-fang,GUAN Yi-xin,YAO Shan-jing.Integrated Applications of Several Separation Methods in the Protein Renaturation[J].Bulletin of Science and Technology,2004,20(2):99-104. |
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| Authors: | CUI Zhi-fang GUAN Yi-xin YAO Shan-jing |
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| Abstract: | To resolve the problems encountered in the protein refolding, separation methods i.e. ultrafiltration, reverse micelle and gel extraction were introduced. Their applications in the process of protein separation and refolding were involved and the mechanism of each process was elucidated. Novel techniques of protein refolding may be expected to emerge with the inspiration of the relatively mature protein separation process. |
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| Keywords: | biochemistry engineering protein renaturation ultrafilration reverse micelle gel extraction |
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