| 复性速率液相分子杂交法测定芽孢杆菌DNA杂交度 |
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| 引用本文： | 梁峰,刘秀花,刘茵. 复性速率液相分子杂交法测定芽孢杆菌DNA杂交度[J]. 商丘师范学院学报, 2000, 16(2): 97-99 |
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| 作者姓名： | 梁峰 刘秀花 刘茵 |
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| 作者单位： | 商丘师专生物系,河南,商丘,476000 |
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| 基金项目： | 河南省科技攻关计划资助项目(991160119) |
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| 摘 要： | 报道采用热变性温度法(Tm)测定枯草芽孢杆菌和短小芽孢杆菌的DNA中G+C mol%分别为42.7mol%和40.26 mol%,与文献报道相一致,说明该法可为细菌分类学提供可靠的分子生物学数据.采用复性速率液相杂交法测定上述两菌DNA-DNA杂交度为9.66%,显示枯草芽孢杆菌与短小芽孢杆菌为同属中相互关系并不十分密切的两个种.
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| 关 键 词： | 芽孢杆菌 复性速率 分子杂交 |
| 文章编号： | 1008-2662(2000)02-0097-03 |
| 修稿时间： | 1999-08-20 |
Determine the DNA binding degree of bacillus subtilis and bacillus pumilus with denaturalization speed in liquid phasic for molecule hybridizition |
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| LIANG Feng,LIU Xiu-hua,LIU Yin. Determine the DNA binding degree of bacillus subtilis and bacillus pumilus with denaturalization speed in liquid phasic for molecule hybridizition[J]. Journal of Shangqiu Teachers College, 2000, 16(2): 97-99 |
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| Authors: | LIANG Feng LIU Xiu-hua LIU Yin |
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| Abstract: | This paper report Bacillus subtilis and Bacillus pumilus DNA G C mol% are 42.7 mol% and 40.26 mol% adopt denaturalization temperature (Tm)methods.The datum closely consistent to literature,and It clew that these datum can used as a credible datum for bacterial taxonomy.Between two strain the DNA-DNA binding degree is 9.66%,with denaturalization speed in liquid phasic for molecule hybridizition,it showed Bacillus subtilis and Bacillus pumilus are two not consanguineous strains in same genus. |
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| Keywords: | bacillus denaturalization speed molecule hybridizition |
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