| 耐高温酒精酵母的筛选及26SrDNA序列鉴定 |
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| 引用本文： | 李,艾.耐高温酒精酵母的筛选及26SrDNA序列鉴定[J].唐山学院学报,2015,28(3):76-79, 82. |
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| 作者姓名： | 李 艾 |
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| 作者单位： | 唐山学院 环境与化学工程系,河北 唐山 063000 |
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| 摘 要： | 将从不同地点采集到的样品分离后得到127株酵母菌,再经过四级筛选得到1株耐高温酒精酵母,将其命名为A27,利用26SrDNA的D1/D2区域序列分析法对其进行分子鉴定,结果显示与报道的酿酒酵母26SrDNA同源性为99%,由此在分子水平上验证了A27菌株为酵母属的酿酒酵母。
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| 关 键 词： | 高温 酒精酵母菌 分离筛选 鉴定 |
| 收稿时间： | 2015/3/17 0:00:00 |
Isolation of High Temperature Resistant Alcohol Yeast and Its Identification of 26SrDNA Sequence |
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| LI Ai.Isolation of High Temperature Resistant Alcohol Yeast and Its Identification of 26SrDNA Sequence[J].Journal of Tangshan College,2015,28(3):76-79, 82. |
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| Authors: | LI Ai |
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| Institution: | Department of Environmental and Chemical Engineering, Tangshan College, Tangshan 063000, China |
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| Abstract: | The author of this paper separated the samples collected from different locations, got 127 yeast strains, then obtained 1 high-temperature alcohol yeast strain after 4 screenings, which was named A27, and finally made a molecular identification of it by using 26 srdna D1/D2 area sequence analysis method. e results reveal that it has 99% homology of Saccharomyces cerevisiae 26SrDNA, thus proving that the strain A27 is saccharomyces cerevisiae at the molecular level. |
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