| 氨基黑10B与蛋白质结合反应的研究 |
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| 引用本文： | 陈冰心,邱艳.氨基黑10B与蛋白质结合反应的研究[J].内江师范学院学报,2011,26(6):29-31. |
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| 作者姓名： | 陈冰心 邱艳 |
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| 作者单位： | 1. 内江市第六中学校,四川内江,641100
2. 内江师范学院化学化工学院,四川内江,641100 |
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| 摘 要： | 在HCl介质中,氨基黑10B与人血清白蛋白（HSA）在室温下能迅速结合生成蓝色缔合物,而使氨基黑10B溶液褪色,其最大吸收峰位于598nm处.人血清白蛋白含量在4.0~20μg/mL范围内基本服从比耳定律,其线性回归方程为ΔA=0.0672c＋0.1137,相关系数为0.9970,表观摩尔吸光系数为2.07×106L.mol-1.cm-1.该方法具有较高灵敏度和良好的选择性,且操作简便快速.
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| 关 键 词： | 氨基黑10B 蛋白质 分光光度法 |
On the Association Reaction of Amido Black 10B and Protein |
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| CHEN Bing-xin,QIU Yan.On the Association Reaction of Amido Black 10B and Protein[J].Journal of Neijiang Teachers College,2011,26(6):29-31. |
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| Authors: | CHEN Bing-xin QIU Yan |
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| Institution: | CHEN Bing-xin1,QIU Yan2 (1.Neijiang No.6 Middle School,Neijiang,Sichuan 641100,China,2.College of Chemistry and Chemical Engineering,Neijiang Normal University,China) |
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| Abstract: | In the medium of HCl,amido black 10B and human serum albumin can rapidly combine and produce blue complex at the room temperature,in the process of which the maximum absorption peak is at 598 nm.Beer＇s law is basically obeyed within the range 4.0～20 μg/mL for human serum albumin with an apparent molar absorptivity of 2.07×106 L·mol-1·cm-1. This method gives a rather satisfactory performance in regards of sensitivity and selectivity and proves to be prompt and easy to handle.. |
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| Keywords: | amido black 10B human serum albumin sectrophotometry |
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