| 自身蛋白作载体制备克伦特罗抗血清 |
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| 引用本文： | 刘见,刘永德.自身蛋白作载体制备克伦特罗抗血清[J].实验室研究与探索,2007,26(3):43-45. |
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| 作者姓名： | 刘见 刘永德 |
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| 作者单位： | 1. 香港科技大学,香港,九龙
2. 上海交通大学农业与生物学院,上海,201101 |
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| 基金项目： | 上海市青少年科技创新基金 |
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| 摘 要： | 本试验以偶氮法将克伦特罗连接到自身全血清和血红蛋白上制成佐剂苗分别免疫兔。免疫后用间接抑制ELISA、间接ELISA检测各组抗体特异性抗克伦特罗效价。结果表明,各组兔血清均含有抗克伦特罗的特异性抗体,显示自身全血清和血红蛋白能作载体,提示自身全血清因组分复杂作载体具有一定优越性。
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| 关 键 词： | 自身蛋白 载体 克伦特罗 抗血清 |
| 文章编号： | 1006-7167（2007）03-0043-03 |
| 收稿时间： | 2006-04-14 |
| 修稿时间： | 2006-04-14 |
Self-proteins of Rabbits as Carriers to Prepare Anti-Clenbuterol Sera |
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| LIU Jian,LIU Yong-de.Self-proteins of Rabbits as Carriers to Prepare Anti-Clenbuterol Sera[J].Laboratory Research and Exploration,2007,26(3):43-45. |
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| Authors: | LIU Jian LIU Yong-de |
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| Institution: | 1. HK Univ. of Science and Technology, Kowloon Hong Kong,China; 2. School of Agricultural and Biology, Shanghai Jiaotong Univ., Shanghai 201101, China |
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| Abstract: | Clenbuterol(CL)was conjugated with rabbit whole sera and rabbit hemoglobin to immunize rabbits respectively.Indirect ELISA and indirect inhibitory ELISA were used to detect the value and specificity of all sera of the immunized rabbits anti-CL IgG.It shows that all rabbits produce anti-CL antibodies,while the sera is not detected out anti-carrier IgG.It suggested that self-whole sera and hemoglobin can be used as carrier,and self-whole sera carriers of complex components are advantageous. |
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| Keywords: | self-proteins carrier Clenbuterol anti-sera |
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