An approach of a study of the interaction between collagen and sulphur dioxide by using ESI and MALDI-TOFMS |
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Institution: | 2. Department of Chemical Engineering, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussel, Belgium |
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Abstract: | In order to further understand the interaction between pollutants and collagen, a study with two plain proteins, bovine serum albumin (BSA) and lysozyme, both exposed to SO2 has been carried out. X-ray analysis of the samples using a scanning microscope spectrometer (SEM) was carried out. Samples were analysed by mass spectrometry using an electrospray source coupled to a time of flight analyser (ESI-TOFMS) and by Fourier transform infrared spectroscopy (FT-IR). A chemical cleavage using cyanogen bromide (CNBr) was carried out on BSA exposed to SO2, the CNBr cleavage fragments were analysed by matrix-assisted laser desorption/ionization source coupling to a TOF analyser (MALDI-TOFMS). All these results allow us to determine a hypothesis for the mechanism of the reaction between SO2 and BSA. |
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