基于共价连接的辣根过氧化物酶的直接电化学与高稳定性传感器构建

利用金电极上自组装的3-巯基丙酸自组装膜,通过DCC和NHS共价连接辣根过氧化物酶（HRP）,构建了一种稳定性好、寿命长的过氧化氢电化学生物传感器.HRP在该电极上实现了直接电子传递,其氧化还原峰电位差为250mV,式量电位为-231 mV.该传感器对过氧化氢表现出良好的电催化活性,还原峰电流与过氧化氢含量在1.5-1000μmol/L之间呈现良好的线性关系,灵敏度达到1185μA/mM,检出限为0.5μmol/L.该传感器具有良好的稳定性、重现性,已用于合成样品中过氧化氢含量的测定,准确度良好.

Direct Electrochemistry Horseradish Peroxides and Highly Stable Hydrogen Peroxide Biosensors Based on Covalent Immobilization on 3-mercaptopropionic Acid Self-assembled Monolayer

A highly stable hydrogen peroxide biosensor has been prepared based on horseradish peroxides （HRP） covalently immobilized on 3--propionic acid self--assembled monolayer by DCC and NHS. Direct electrochemistry of HRP has been achieved with redox peak separation of 250 mV and formal potential of --231 inV. The proposed biosensor exhibits better electrocatalysis towards hydrogen peroxide, the linear range is 1.5--1000 μmol/L with sensitivity of 1185 μA/mM and detection limit of 0.5 μmol/L. The proposed biosensor shows its high stability, reproducibility and anti--interference, and has been used to determine hydrogen peroxide in synthesized samples with satisfactory results.