均匀设计法优选鸡血藤总黄酮提取工艺研究

目的：优选鸡血藤总黄酮提取的最佳工艺条件。方法：利用紫外分光光度法测定总黄酮的含量，以鸡血藤总黄酮含量作为评价指标，采用U（94）均匀设计表设计试验，观察与分析提取剂浓度、提取时间、料液比、颗粒度4种因素对鸡血藤总黄酮提取的影响。结果：提取鸡血藤总黄酮最佳状态是：60％的乙醇，鸡血藤粉碎程度50目，液料比为40：1，提取药材时间2.5小时，结论：本工艺操作简便合理，结果稳定可靠，重复性好，具有可行性，可推广应用。

Total Flavonoids Extraction Process Analysis of Net Cliffbean by Uniform Design

Objective： To optimize the extraction process of total flavonoids in Net Cliffbean. Methods： UV were established to determine total flavonoids, U （94） Uniform design form was selected as the experiments＇ schedule, the total flavonoids was used as evaluation indicator to and the extraction solvent concentration, extraction time, solid - liquid ratio and particle size was used as the 4 influencing factors. Results： The uniform design experiment show that 60% ethanol concentration, particle degree of 50 orders, the liquid -solid ratio of 40 ： 1 and the extraction time of 2.5 hours were the optimum extraction process. Conclusions： The method can quickly, accurately determine the optimum extraction technology of flavonoids from Net Cliffbean.