| 商陆小G蛋白激活蛋白基因 PaAGAP 在逆境下表达研究 |
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| 作者姓名： | 刘芳 吴亮其 王辉 张媛雅 柴团耀 |
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| 作者单位： | 1. 中国科学院研究生院,北京 100049;
2. 中国矿业大学,北京100083 |
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| 基金项目： | 国家高技术研究发展计划(863)项目(2006AA10Z407, 2007AA021404)和中国科学院研究生院院长基金B类项目(085102FN00)资助 |
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| 摘 要： | 利用同源克隆和RACE方法在商陆( Phytolacca acinosa )中得到一个推测的小G蛋白ArfGTPase激活蛋白 ArfGAP (ArfGTPase activating protein) 的全长cDNA序列 PaAGAP . PaAGAP 序列编码332个氨基酸.蛋白含有保守的锌指结构域(CX2CX16CX2C类)和C2结构域.实时荧光定量PCR表明在寒、旱、盐和重金属的不同时间胁迫下,PaAGAP可以被诱导,表达量有不同程度的上调.由此推测 PaAGAP 可能是通过快速启动转录和翻译,使ARF失活,抑制植物生长素极性运输,来参与植物逆境反应的.
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| 关 键 词： | 商陆 ArfGAP 锌指 逆境 表达 |
| 收稿时间： | 2010-04-09 |
| 修稿时间： | 2010-04-23 |
Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress |
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| Authors: | LIU Fang WU Liang-Qi WANG Hui ZHANG Yuan-Ya CHAI Tuan-Yao |
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| Institution: | 1. College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
2. China University of Mining & Technology, Beijing 100083, China |
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| Abstract: | A full-length cDNA of an ArfGAP ( PaAGAP ) encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE. PaAGAP is predicted to contain a CX2CX16CX2C type zinc finger domain and a C2 domain. Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature, osmotic, salt, and heavy metal stresses, indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport. |
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| Keywords: | Phytolacca acinosa ArfGAP zinc finger stress expression |
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