| RACE技术的研究进展及其应用 |
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| 引用本文： | 陈启龙. RACE技术的研究进展及其应用[J]. 黄山学院学报, 2006, 8(3): 95-98 |
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| 作者姓名： | 陈启龙 |
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| 作者单位： | 黄山学院,生物系,安徽,黄山,245021 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | cDNA末端快速扩增(RACE)技术是一种基于PCR反应的技术,它的的发展极大地方便了克隆全长cDNA3′端和5′端的工作。本文综述了RACE技术的原理、局限性及改进方法,以及RACE技术在基因表达方面的研究进展和应用前景。
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| 关 键 词： | RACE cDNA全长 |
| 文章编号： | 1672-447X（2006）03-0095-04 |
| 收稿时间： | 2005-10-17 |
| 修稿时间： | 2005-10-17 |
Development of Rapid Amplification of cDNA Ends and Its Application |
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| Chen Qilong. Development of Rapid Amplification of cDNA Ends and Its Application[J]. Journal of Huangshan University, 2006, 8(3): 95-98 |
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| Authors: | Chen Qilong |
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| Affiliation: | Chen Qilong |
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| Abstract: | Rapid Amplification of cDNA Ends(RACE)is a PCR-based technique which was developed to facilitate the cloning of full-length cDNA 3' and 5' ends.This paper reviews the principle,shortcoming and improvements of RACE.The perspective and application of RACE for the expression of genes is also reviewed. |
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| Keywords: | RACE full-length cDNA |
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