A novel way of liver preservation improves rat liver viability upon reperfusion |
| |
Authors: | Kebis Anton Kukan Marián Gran?i? Peter Jakubovský Ján |
| |
Institution: | (1) Laboratory of Perfused Organs, Slovak Center for Organ Transplantation, Slovak Medical University, Limbová 12, 83303 Bratislava, Slovakia;(2) Department of Pathology, School of Medicine, Comenius University, 81372 Bratislava, Slovakia |
| |
Abstract: | Background/aim Currently, the liver is cold-preserved at 0∼4 °C for experimental and clinical purposes. Here, we investigated whether milder
hypothermia during the initial phase of the preservation period was beneficial for liver viability upon reperfusion.
Methods In the first set of experiments, rat livers were preserved either conventionally in clinically used histidine-trypthopan-ketoglutarate
(HTK) solution (Group A: 45 min and Group B: 24 h) or by slow cooling HTK solution (from 13 °C to 3 °C) during the initial
45 min of preservation (Group C: 24 h). In the second set of experiments, additional groups of livers were evaluated: Group
BB—preservation according to Group B and Group CC—preservation according to Group C. Further, some livers were preserved at
13 °C for 24 h. Livers were then reperfused using a blood-free perfusion model.
Results Bile production was approximately 2-fold greater in Group C compared to Group B. Alanine transaminase (ALT) and aspartate
transaminase (AST) release into perfusate were 2∼3-fold higher in Group B compared to Group C. No significant differences
were found in ALT and AST release between Group C and Group A. Livers in Group CC compared to Group BB exhibited significantly
lower portal resistance, greater oxygen consumption and bromosulfophthalein excretion into bile and lower lactate dehydrogenase
(LDH) release into perfusate. Histological evaluation of tissue sections in Group BB showed parenchymal dystrophy of hepatocytes,
while dystrophy of hepatocytes was absent in Group CC. Livers preserved at 13 °C for 24 h exhibited severe ischemic injury.
Conclusion These results suggest that the conventional way of liver preservation is not suitable at least for rat livers and that slow
cooling of HTK solution during the initial phase of cold storage can improve liver viability during reperfusion.
Project supported by the Ministry of Health of the Slovak Republic under the project of Modulation of Heat Transfer in Isolated
Liver (No. 2005/32-SZU-10) and the VEGA Grant (No. 1/1158/04), Slovakia |
| |
Keywords: | Rat Hepatic graft Cold ischemia Liver protection Histidine-trypthopan-ketoglutarate solution (HTK) |
本文献已被 维普 PubMed SpringerLink 等数据库收录! |
|