胡桃属十种植物的过氧化物同工酶分析

用丙烯酰胺凝胶电泳分离了胡桃属Juglans L．10种植物80份材料的过氧化物同工   酶，共出现16种酶谱。  种内酶谱多态，但酶带间的差异小于种间酶带差异。用“酶谱距离”   及特异酶带可将10个种分成4组，与胡桃属分类基本吻合。  黑核桃组的酶带最多。胡桃楸   次之，核桃及灰核桃最少。根据同工酶的产生与进化的关系，可认为黑核桃与胡桃楸比核桃、  灰核桃进化。胡桃属内平均“酶谱距离”为0.52，推测该属应发生在晚白垩纪到古新世。

A Study on Isozymes of Peroxidase of 10 Species in Juglans L.

 Isozymes of peroxidase of 10 species in Juglans L. were analyzed by using poly- crylamide gel electrophoresis, and, as a result, 16 different patterns of isozymes were observed. Polymophism of isozymes patterns appears within species and more significant differences in pat- tern between species have been found. “Zymogram distance” was measured for each species pair and section pair.  The ten species may be divided into 4 groups according to their “zymo- gram distance” and specific bands, which accords with the classical taxonomy of Juglans L.. Evolutionary relationship among species and rate of evolution for Juglans L. are discussed.