Targeting of human aFGF gene into silkworm,Bombyx mori L. through homologous recombination

INTRODUCTION Transgenic technology developed over the 20years, has become a common tool widely applied tolivestock species because it is technically quitesimple, requiring only the injection of ‘naked’DNA into the nucleus of a fertilized eggs (Hammeret al., 1985; Suraokar and Bradley, 2000). Howeverthis technique has limitations in that the injectedDNA integrates randomly into the genome and thuscould not be expressed in the desired tissue or at theappropriate level. More impor…

Targeting of human aFGF gene into silkworm,Bombyx mori L. through homologous recombination

The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through ho- mologous recombination.