| 一株产表面活性剂的高效烃降解菌的筛选 |
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| 引用本文： | 马飞,潘科,陶文,马莉莎,郭峰.一株产表面活性剂的高效烃降解菌的筛选[J].茂名学院学报,2012(1):22-25. |
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| 作者姓名： | 马飞 潘科 陶文 马莉莎 郭峰 |
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| 作者单位： | 长江大学油气资源与勘探技术教育部重点实验室;长江大学地球化学系;中国石油吐哈油田分公司;中国石油青海油田分公司勘探开发研究院 |
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| 摘 要： | 以大庆油田污染土壤为对象,通过血平板、油平板、摇瓶发酵筛选出了1株高效烃降解菌M。原油摇瓶发酵实验表明原油的降解率为57%。该菌株能够产生大量的活性物质,经傅里叶红外光谱分析表明其产生的生物活性物质为脂肽类化合物,该脂肽类生物表面活性剂理化性质显示它能使发酵液的表面张力从56.1 mN/m（CK）降低为32.3mN/m。排油实验、油水乳化稳定性测定表明该菌产生的表面活性剂能具有较强的乳化原油的能力,展现了较大的应用潜力。
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| 关 键 词： | 烃降解菌 生物修复 烃降解率 生物表面活性剂 |
Isolation of A Hydrocarbon Degrading Bacteria and Biosurfactants-Producing Bacteria |
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| MA Fei,PAN Ke,TAO Wen,MA Li-sha,GUO Feng.Isolation of A Hydrocarbon Degrading Bacteria and Biosurfactants-Producing Bacteria[J].Journal of Maoming College,2012(1):22-25. |
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| Authors: | MA Fei PAN Ke TAO Wen MA Li-sha GUO Feng |
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| Institution: | 1,2(1.Key Laboratory of Petroleum Resources and Exploration Technology,Yangtze University,Jingzhou 434023,China;2.Department of Geochemistry,Yangtze University,Jingzhou 434023,China;3.Research Institute of Petroleum Exploration and Development,Qingha i Oilfield Company,Dunhuang 736200,China;4.Tuha Oilfield Company,Petro China,Tulufan 83800,China) |
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| Abstract: | In order to manage the problem of petroleum contaminated soil effectively,a hydrocarbon degrading bacteria and Biosurfactants-Producing Bacteria M isolated from oil contaminated soil in Daqing oil field,.Petroleum hydrocarbon degradation was researched in this thesis.The experiments of degrading crude oil indicated that the bacteria of M had a powerful ability and the biodegradation rate of TPH came to 57% after 7 d.The bio-surfactants produced by the bacteria of M were mainly indentified as glycolipid by FT-IR analysis.The bio-surfactants produced by the bacteria of M made the surface tension of the medium reduce from 56.1 mN m-1(CK) to 32.3 mN m-1.The study reported that the bacteria have a high oil degradation ability and could decrease surface tension effectively,with large potential application. |
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| Keywords: | petroleum hydrocarbon degradation bacteria bioremediation rate of Total Petroleum Hydrocarbon(TPH) bio-surfactant |
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