| 白头翁高效液相色谱指纹图谱研究 |
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| 引用本文： | 方艳夕,潘勇,秦梅颂,周丽丽,朱金坤,时维静.白头翁高效液相色谱指纹图谱研究[J].宜春学院学报,2011,33(12). |
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| 作者姓名： | 方艳夕 潘勇 秦梅颂 周丽丽 朱金坤 时维静 |
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| 作者单位： | 安徽科技学院食品药品学院,安徽滁州,233100 |
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| 基金项目： | 安徽省高校省级自然科学研究项目 |
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| 摘 要： | 目的：建立白头翁药材高效液相色谱指纹图谱，并比较了不同产地白头翁中白头翁皂苷B。含量的差异。方法：采用Agilent ZORBAXSBC18（4．6mm×250mm，5μm）色谱柱；乙腈（A）和水（B）为流动相，流速1．0ml／min；柱温40oc；检测波长201nm。结果：十批白头翁样品高效液相色谱指纹图谱具有一定的相似性；不同产地白头翁中白头翁皂苷B4。的含量有差异。结论：本法准确、可靠，建立的白头翁高效液相色谱指纹图谱可结合白头翁皂苷B4。含量测定用于白头翁药材的质量控制。
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| 关 键 词： | 白头翁 高效液相色谱 指纹图谱 白头翁皂苷B4 |
Study on the HPLC Fingerprint of Pulsaflllae Radix |
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| FANG Yan-xi,PAN Yong,QIN Mei-song,ZHOU Li-li,ZHU Jin-kun,SHI Wei-jing.Study on the HPLC Fingerprint of Pulsaflllae Radix[J].Journal of Yichun University,2011,33(12). |
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| Authors: | FANG Yan-xi PAN Yong QIN Mei-song ZHOU Li-li ZHU Jin-kun SHI Wei-jing |
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| Institution: | FANG Yan-xi,PAN Yong,QIN Mei-song,ZHOU Li-li,ZHU Jin-kun,SHI Wei-jing(Anhui Science and Technology University,College of Food and Drug,Chuzhou 233100,China) |
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| Abstract: | Objective： To establish the HPLC fingerprint and compare the content of pulchinenoside B4 of Pulsatillae Radix from different areas. Methods： RP - HPLC method was applied and the conditions were as follows： Column： Agilent ZORBAX SBCiscolumn （4.6mm×250 mm, 5 μm）; mobile phase： acetonitrile （A） and H20 （B）, flow-rate： 1.0 mL/min; column temperature： 40℃ ; the detection wavelength was 201nm. Results： There was a certain similarity in the fingerprint of the ten samples of Pulsatillae Radix. The difference on the content of pulchinenoside B4 was obvious. Conclusion： The method is accurate, reliable and the fingerprint can be combined with content determination of pulchineneside B4 in the quality~control of Pulsatillae Radix. |
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| Keywords: | Ptdsatillae Radix High performance liquid chromatography Fingerprint Pulchinenoside B4 |
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